北京百奧創(chuàng)新科技有限公司提供Gibco FreeStyle™ F17表達(dá)培養(yǎng)基現(xiàn)貨，更多Gibco培養(yǎng)基的介紹，歡迎咨詢(xún)！

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更新時(shí)間：2024-12-06

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產(chǎn)品介紹

一、產(chǎn)品簡(jiǎn)介

產(chǎn)品中文名稱(chēng)：Gibco FreeStyle™ F17表達(dá)培養(yǎng)基

貨號(hào)：A1383501

產(chǎn)品描述：

Features:

• Little or no required adaptation

• Supports suspension growth and transfection of HEK293 cells

• Chemically defined, protein-free, serum-free formulation

• Compatible with multiple transient production systems

• Scalability in spinner flasks and bioreactors

Little or no required adaptation:

293-F, 293-H, and FreeStyle 293-F cells in suspension can be cultured directly into FreeStyle F17 Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into FreeStyle F17 medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.

Growth and transfection of HEK293 cells:

FreeStyle F17 Expression Medium supports 293-F cells at a peak density of 6–7 x 106 viable cells per mL. Cells cultured in FreeStyle F17 medium can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin and Freestyle MAX Reagent.

Scalability in spinner flasks and bioreactors:

Production using FreeStyle F17 Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement FreeStyle F17 medium with additional Pluronic F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system:

FreeStyle F17 Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

產(chǎn)品應(yīng)用：For research use only.

二、代理簡(jiǎn)介

Gibco FreeStyle™ F17表達(dá)培養(yǎng)基代理——北京百奧創(chuàng)新科技有限公司：

（一）輻射五大服務(wù)方向：

1.國(guó)內(nèi)試劑耗材經(jīng)銷(xiāo)代理；

2.國(guó)外試劑訂購(gòu)（直訂，或境外代理）；

3.基因組測(cè)序（二代，二代+三代）；

4.基因分型（SNP檢測(cè)）：低/中/中高通量項(xiàng)目；

5.生物樣本運(yùn)輸（國(guó)內(nèi)、）；

（二）多家代理品牌，涉及30多種較有名品牌，經(jīng)營(yíng)代理500多個(gè)品牌：

Qiagen、Sigma、R&D、Epigentek、ThermoFisher、DNA Genotek、USA Scientific、

Matreya、Sage Science、List Labs、Macrocyclics、Steraloids、